The best Side of attune flow cytometer
The best Side of attune flow cytometer
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At the moment, the early symptoms have been dismissed as particles, nevertheless the retrospective evaluation demonstrated shared properties With all the problematic cells in culture. Tracing the an infection served the lab create additional laboratory techniques for screening and protection of assay-significant mobile strains.
As samples are acquired within the Attune CytPix Flow Cytometer, the high-velocity brightfield digicam captures and merchants illustrations or photos of detected situations in a fee nearly six,000 photographs for every second, according to the flow level and graphic dimensions. For greater overall flexibility, Attune Cytometric Software program permits you to adjust the image seize frequency as necessary and choose a specific gate for graphic seize, if demanded.
Merge acoustic concentrating know-how with high-parameter analysis for versatile spectral unmixing and traditional workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing which has a higher-velocity brightfield digicam for simultaneous significant throughput flow cytometry and superior resolution brightfield imaging.
"A picture just isn't pretty well worth 1000 dots, nonetheless it actually helps you to see illustrations or photos. It is excellent to find out images that correlate with staining. One example is, AnnexinV-constructive cells which might be morphologically distinct than healthier cells, or CD14+ monocytes which have been bigger plus more textured than CD3+/CD4+/CD14- lymphocytes.
Moreover, it's illuminating to determine the number of doublets slip as a result of traditional singlet gates, and wonderful to confirm the position of unforeseen double-beneficial activities in my Investigation."
Pair your Attune flow cytometer using an autosampler for enhanced workflow efficiency, and incorporate robotic integration for max throughput.
Our researchers will design panels in your case as again and again you'll need—totally free, no merchandise obtain desired.
Early signs of this contamination had to begin with been dismissed as debris. Backgating to discover new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also lets you use morphological attributes to discover exciting subpopulations that will not here be evident from flow cytometry info by itself.
Potentially most significantly, these functions comprise cells of Obviously distinctive phenotypes which could lead to incorrect conclusions with regards to double favourable activities (especially in scarce populations).
Sound waves allow specific mobile alignment, leading to Outstanding knowledge excellent and ~10X faster sample flow costs.
Application Observe: Automated impression analysis cuts down user-to-user variability in flow cytometry gating techniques
Crafted-in dimensionality reduction equipment elevated resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to confirm predicted populace resolution in lysed complete human blood. User can check out functions from Every single cluster for Visible confirmation of mobile phenotypes.
Flexible payment prepare from Agilent Money Answers help you receive the critical instrumentation you require for fast task, or to develop your lab.
At the guts of each and every Attune Flow Cytometer lies our revolutionary acoustic concentrating know-how, which makes use of sound waves to meticulously align cells within the flow mobile. This progressive strategy surpasses regular hydrodynamic strategies, causing more rapidly processing speeds, Outstanding facts high quality, and improved sample throughput.
The Attune Xenith cytometer is really a superior-parameter spectral analyzer, offering equally spectral unmixing and standard flow capabilities for detailed and functional cell Assessment.
Agilent Price Guarantee assures you a minimum of ten years of instrument use with the day you purchase. Otherwise, we will credit history you Using the residual value of the program toward an upgraded design.
What seemed to be a homogeneous inhabitants is really far more numerous—an Perception that needs to be considered when interpreting benefits.